OrangerV1, France, Analysis, bibRecord, 000172

Purification, molecular cloning, and cell-specific gene expression of the alkaloid-accumulation associated protein CrPS in Catharanthus roseus

Identifieur interne : 000172 ( France/Analysis ); précédent : 000171; suivant : 000173

Purification, molecular cloning, and cell-specific gene expression of the alkaloid-accumulation associated protein CrPS in Catharanthus roseus

Auteurs : Diane Leme Nager [France, Espagne] ; Lazhar Ouelhazi [France, Tunisie] ; Samira Mahroug [France] ; Bertrand Veau [France] ; Benoit St-Pierre [France] ; Marc Rideau [France] ; Jone Aguirreolea [Espagne] ; Vincent Burlat [France] ; Marc Clastre [France]

Source :

Journal of Experimental Botany [ 0022-0957 ] ; 2005-04.

RBID : ISTEX:F53C5249FD225A76AAD57081DD49169AA5A01B8F

English descriptors

KwdEn :
- Catharanthus roseus, in situ RNA hybridization, monoterpenoid indole alkaloid, phytohormone, two-dimensional gel electrophoresis, α/β hydrolase.

Abstract

Identification of molecular markers of monoterpenoid indole alkaloid (MIA) accumulation in cell-suspension cultures of Madagascar periwinkle (Catharanthus roseus (L.) G. Don) was performed by two-dimensional polyacrylamide gel electrophoresis. Comparison of the protein patterns from alkaloid-producing and non-producing cells showed the specific occurrence of a 28 kDa polypeptide restricted to cells accumulating MIAs. The polypeptide was purified by preparative two-dimensional gel electrophoresis, digested with trypsin, and microsequenced by the Edman degradation method. Cloning of the corresponding cDNA revealed that the protein which has been named CrPS (Catharanthus roseus Protein S) is a member of the α/β hydrolase superfamily. Time-course expression studies by northern blot analysis confirmed that CrPS gene expression was associated with MIA accumulation in cell suspension cultures. In the whole plant, multicellular compartmentation is required for alkaloid biosynthesis. In situ mRNA hybridization on developing leaves revealed that CrPS mRNA and transcripts encoding the first enzymes of the MIA pathway were co-localized in internal phloem parenchyma cells. The possible implication of the alkaloid-accumulation associated protein CrPS in the signal transduction pathway leading to MIA production is discussed.

Url:

https://api.istex.fr/document/F53C5249FD225A76AAD57081DD49169AA5A01B8F/fulltext/pdf

DOI: 10.1093/jxb/eri116

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream Istex, to step Checkpoint: 000756
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to stream Main, to step Curation: 002770
to stream Main, to step Exploration: 002770
to stream France, to step Extraction: 000172

Links to Exploration step

ISTEX:F53C5249FD225A76AAD57081DD49169AA5A01B8F

Le document en format XML

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<author><name sortKey="Veau, Bertrand" sort="Veau, Bertrand" uniqKey="Veau B" first="Bertrand" last="Veau">Bertrand Veau</name>
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<author><name sortKey="Aguirreolea, Jone" sort="Aguirreolea, Jone" uniqKey="Aguirreolea J" first="Jone" last="Aguirreolea">Jone Aguirreolea</name>
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<author><name sortKey="Burlat, Vincent" sort="Burlat, Vincent" uniqKey="Burlat V" first="Vincent" last="Burlat">Vincent Burlat</name>
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<front><div type="abstract" xml:lang="en">Identification of molecular markers of monoterpenoid indole alkaloid (MIA) accumulation in cell-suspension cultures of Madagascar periwinkle (Catharanthus roseus (L.) G. Don) was performed by two-dimensional polyacrylamide gel electrophoresis. Comparison of the protein patterns from alkaloid-producing and non-producing cells showed the specific occurrence of a 28 kDa polypeptide restricted to cells accumulating MIAs. The polypeptide was purified by preparative two-dimensional gel electrophoresis, digested with trypsin, and microsequenced by the Edman degradation method. Cloning of the corresponding cDNA revealed that the protein which has been named CrPS (Catharanthus roseus Protein S) is a member of the α/β hydrolase superfamily. Time-course expression studies by northern blot analysis confirmed that CrPS gene expression was associated with MIA accumulation in cell suspension cultures. In the whole plant, multicellular compartmentation is required for alkaloid biosynthesis. In situ mRNA hybridization on developing leaves revealed that CrPS mRNA and transcripts encoding the first enzymes of the MIA pathway were co-localized in internal phloem parenchyma cells. The possible implication of the alkaloid-accumulation associated protein CrPS in the signal transduction pathway leading to MIA production is discussed.</div>
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Purification, molecular cloning, and cell-specific gene expression of the alkaloid-accumulation associated protein CrPS in Catharanthus roseus

Purification, molecular cloning, and cell-specific gene expression of the alkaloid-accumulation associated protein CrPS in Catharanthus roseus

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri